Protein Details: Chloride channel protein clh-3

Protein ID

ICDB_Pro_1454

Protein Name

Chloride channel protein clh-3

Gene Name

clh-3; clc-3; E04F6.11

Organism

Caenorhabditis elegans

Length

1001 amino acids

AlphaFoldDB

AF-Q9BMK9-F1-model_v4.pdb

Function

[Isoform a]: Voltage-gated chloride channel . Insensitive to depolarizing conditioning voltages; requires low voltages for activation; insensitive to chloride levels and has a mild sensitivity to low pH. Channel gating properties are conferred by the cytoplasmic C-terminus . Plays a role in egg laying by modulating hermaphrodite-specific neurons (HSN) excitability and the ovulatory contractions of gap-junction-coupled gonadal sheath cells . When active; may prevent tubular formation of the excretory canals . Activated during oocyte meiotic maturation and by membrane hyperpolarization and cell swelling . Inhibited by Zn(2+) and to a lesser extent by Cd(2+).; [Isoform b]: Voltage-gated chloride channel . Sensitive to depolarizing conditioning voltages; requires stronger voltages for activation and activation is slower; is inhibited by low concentrations of chloride and is activated by low pH. Channel gating properties are conferred by the cytoplasmic C-terminus .

Sequence

MGIGTKILSKIEKNKTSDGLTIPLTPTTQKQSSSWCSFESIKTFFRTVIRDWIFLALLGFIMASLSFGMDYAILNLQNGQMRLFDLVKEYHFTLAYLVWVGYVVGLILLSAVCAHYIAPQAIGSGIPEMKTILRGVILKEYLSVRTLLSKMIGLTLSLGSGLPMGKEGPFVHVASVVASQLTRLVHGSSGGIFENESRSGEMLAAGCAVGVACTFSAPIGGVLFSIEVTSVYFAVRNYWRGFFAATCSATLFRILRMFSVSAAVTVEAHYQTNFPPQNVFLPQELPIFALIGLVCGLAGSIFVYLHRRTVLFLRRNWLAKMIFQKYWLIYPIFIATFISSLSFPLGLGKFMGGEERFSHTMKEFFVDCAWTAPPNDSYACPMPTSNATSSDSFDIRHWKGDNYDYSPFVTLSSFQVVYFFLAILASTLPVPSGIFMPVFVLGAAFGRLVGEGVFSLDPYGHISGDIQFFVRPGVYAVVGAAAFCGAVTHTVSVAVIVFELTGQLCHLLPVMIAVLIANAVASYLQPSIYDSIIRIKNLPYLPDIPHTTSLYHQMLIEQFMISPLVYIAKDSTVGDIKRALETKTRIRAFPLVENMESLALVGSVSRSQLQRYVDSQIGTKARFAEATRRIKQRLEDEESERKRREESKSDDTEDSLETTGAGERRASRFLIVPVAKNGPQVAKNETLTGLSEENARKILTVEEKQALFDAASLATPKREMSGKTINPVHIESHHTIGDIFRSITHLSFGRQNFPKKNNHNEFDLFGEERTEWEDMMLNQKLDLSQLDIDSTPFQLSEYTSLFKAHSLFSLLGLNRAYVTKKGQLIGVVALKELRLAIEYLQSGKVPTPGMSIFNEPPTEQSIYEKSARLESGRATGDAQNAAFVTDNGEDDAQNDYIQPPLEVVRRGALTPNRMSELTRLENVRTTPESPHFEVSSPSTSSSCVSIDFSPLDAANSENGSVGGLVLNVPSLPTRARSANELTRQNTHVQINLPDDVHDEKF

PDB Structures

Ligand Binding

1. DICL_CP

2. DICL_Pep

Binding Site

BINDING 124; /ligand="chloride" ; BINDING 434; /ligand="chloride" ; BINDING 435; /ligand="chloride" ; BINDING 529; /ligand="chloride"

Disease

Location

Expressed in excretory cell; 4 anterior epithelial cells of the intestine; hermaphrodite-specific neurons and enteric muscles (PubMed:11078724; PubMed:10567397). Expressed also in vulva and uterus. Isoform a is expressed in oocytes (at protein level). Isoform a is expressed in oocytes (at protein level). Isoform a is expressed in oocytes (at protein level).

DOI ID

10.1074/jbc.274.48.34238; 10.1152/ajpcell.2000.279.6.c2052; 10.1126/science.282.5396.2012; 10.1016/s0960-9822(01)00051-3; 10.1083/jcb.200204142; 10.1113/jphysiol.2003.053165; 10.1085/jgp.200409215; 10.1529/biophysj.105.078295; 10.1038/sj.embor.7401128; 10.1085/jgp.200810080; 10.4161/chan.4.4.12445; 10.1016/j.bpj.2012.09.001; 10.1523/jneurosci.3112-13.2014

RefSeq

NP_001022060.1 [Q9BMK9-1]